In Vivo Analysis of Aspergillus fumigatus Developmental Gene Expression Determined by Real-Time Reverse Transcription-PCR
نویسندگان
چکیده
منابع مشابه
In vivo analysis of Aspergillus fumigatus developmental gene expression determined by real-time reverse transcription-PCR.
Very little is known about the developmental stages of Aspergillus fumigatus during invasive aspergillosis. We performed real-time reverse transcription-PCR analysis on lung samples from mice with invasive pulmonary aspergillosis to determine the expression of A. fumigatus genes that are expressed at specific stages of development. In established infection, A. fumigatus exhibited mRNA expressio...
متن کاملReal-Time Reverse Transcription PCR
Real-time, fluorescence-based reverse transcription polymerase chain reaction (RT-PCR) has been transformed from an experimental technology into a mainstream scientific tool for the detection of RNA. This is because of several factors: 1) it is a homogeneous assay, which eliminates the requirement for post-PCR processing; 2) it has a wide dynamic range; 3) there is little interassay variation; ...
متن کاملTargeted gene-expression analysis by genome-controlled reverse transcription-PCR.
BACKGROUND For gene-expression analysis, which is anticipated to play an important role in classification of tumors and premalignant conditions, PCR-based quantitative assays must have increased diagnostic quantitative accuracy and reproducibility and enable analysis of gene expression in formalin-fixed paraffin-embedded (FFPE) tissue samples. METHODS We developed a reverse transcription-PCR-...
متن کاملeffect of phosphatidylcholine on pld gene expression level of aspergillus fumigatus by the real time pcr method and investigations of these genes using bioinformatics analysis.
background and objectives: phospholipases are a group of enzymes that breakdown phospholipid molecules producing second products. these second products play a diverse role in the cell such as signal transduction and digestion in humans. in this study, the effect of phospholipids on the expression of pld genes of a. fumigatus was investigated. the pld genes of this fungus were also investigated ...
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systemic lupus erythemathosus is an autoimmune disease that affected many various types of tissues in 10% of world population and over 30 genes has associated with it. nouroimmunoendocrynology concepts have shown that immune system could be affected by neuron system and vice versa, 5-hydroxytryptamine receptor a (5ht3ra) was studied as a main receptor in these relations. in this study, peripher...
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ژورنال
عنوان ژورنال: Infection and Immunity
سال: 2008
ISSN: 0019-9567,1098-5522
DOI: 10.1128/iai.01483-07